Fig. 3

IDH2 inhibition suppress tumor growth and migration in GH-PA. a IDH2 mRNA levels were 103 ± 21.5%, 77.4 ± 15.2%, 21.3 ± 11.7% and 16.2 ± 8.3% of that in the control group, respectively, after RNAi in GH3 cells. b IDH2 protein levels were identified to be decreased after using shRNA C plasmid and shRNA D plasmid. c IDH2 protein levels were 115 ± 21.0%, 87.0 ± 15.0%, 41.3 ± 12.0% and 26.0 ± 8.0% of that in the control group, respectively, after RNAi in GH3 Cells. d The cell viability was reduced to 91.5 ± 7.1%, 77.2 ± 6.8% and 71.8 ± 7.3% after 24 h, 48 h and 72 h of shRNA C plasmid blockage and 83.9 ± 7.6%, 72.4 ± 8.1% and 64.6 ± 5.9% after 24 h, 48 h and 72 h of shRNA D plasmid blockage in GH3 cells. e The average number of migrated cells was reduced to 254 ± 32 and 226 ± 27 from 456 ± 84 after shRNA C and D plasmid blockage in GH3 cell lines. All assays were performed in triplicate. **Compared to control, p < 0.01. *Compared to control, p < 0.05