Fig. 3
From: Cryopreserved mesenchymal stem cells regain functional potency following a 24-h acclimation period
Gene expression of MSCs from the 3 different groups. Gene expression analysis revealed a downregulation in the tissue injury-related genes, HMGB1 and TLR-4 in FT MSCs. Apoptosis-related genes (cytochrome C, BAX, BCL-2) and the pro-inflammatory TNF-α were downregulated in both TT and FT MSCs. The anti-inflammatory/anti-oxidant genes HMOX and TSG-6 as well as the angiogenesis gene VEGF were upregulated in TT cells. Finally, the stem cell genes, OCT-4, NANOG, and SOX2 as well as the anti-inflammatory gene, STC-1 were downregulated in both TT and FT cells. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001