Fig. 7

In vivo study: human CD45+ cells engraftment in the xenogeneic GVHD murine model. Animals were bled after 4/7 weeks and the percentages of human CD45+ cells in blood was determined by flow cytometry. a Representative dot plots of human CD45+ cell engraftment at 4 weeks after transplant are shown. b, c No human CD45+ cells were identified in the PB of mice after infusion of Tregs alone from the KT or LT patient. Conversely, a wide distribution of human CD45+ cells were observed in the PB of mice after transplant of KT/LT CD8−CD25− T cells alone or CD8−CD25− T cells plus Treg infusion. In addition to individual data, mean values and SEM are shown