Fig. 4

Angiogenic effect of culture supernates. a MSCs and S-MSCs culture supernates and cell-free media (CFU-F and EGM-2) were recovered at day 28. HMEC-1 were suspended in endothelial cell growth medium MV (n = 3), in cell-free CFU-F medium (n = 3), in cell-free EGM-2 medium (n = 3), in MSCs culture supernates (obtained from 5 CLI-MSCs, n = 2 each group), in S-MSCs culture supernates (obtained from 5 CLI-S-MSCs, n = 2 each group) and incubated on Matrigel during 24 h. The extend of the network of the capillary-like tubes was appreciated at 3:30 h. b Quantification of the loops number at 3:30 h. c Quantification of total tube length (µm) at 3:30 h. x: aberrant distribution values as indicated by Box and Whiskers plots Medcalc version 7.3 software (*p < 0.05, ^†p < 0.01 and ^‡p < 0.001)