Fig. 1

Study design. a BMCs were obtained from 7 CLI patients. MSCs were selected and expanded in a CFU-F medium for 28 days (n = 7). Stimulated MSCs were cultured in EGM-2 medium for 14 days (n = 7). b Hindlimb ischemia was induced by femoral artery ligation in Nude mice. Cells (BMCs, MSCs, S-MSCs) or vehicle were injected in the gastrocnemius. Hindlimb perfusion, Necrose detection and functional tests were performed during 28 days. At day 28 muscles were harvested for biological analysis