Fig. 3

Michaelis Menton modeling of MSC hydrolysis. a–e Rates for each population size were determined as a function of dose by finding the slopes of hydrolysis against time graphs. Using non-linear regression fitting, rates as a function of dose were fit to first-order enzyme kinetic equations, which show that Vmax and Km increase as population size increases. For all models, R² > 0.95 and n = 3. f There is a linear relationship between Vmax and normalized cell population, of which the slope determines the maximum hydrolysis rate per cell