Fig. 2

Evaluation of different immunization schedules and duration of T-cell responses after vaccination with NP mRNA. a–c BALB/c mice were immunized according to different vaccination schedules with varying amounts of mRNA NP. In vivo antigen-specific lysis of NP peptide-loaded target cells was evaluated in lymph nodes and spleens 4 or 5 days after the last immunization. d Duration of T-cell responses in mice immunized twice with 17 µg of NP mRNA with 1 week between vaccinations according to schedule 3. Antigen-specific lysis of NP peptide-loaded target cells was assessed by flow cytometry and IFN-γ production was assessed by ELISPOT after boost vaccination on the indicated days. Results are shown as median (a–c) or median ± IQR (d) with each symbol representing one mouse for a total of 4–10 mice per experiment. Differences between groups were analyzed by Kruskal–Wallis test with Bonferroni correction