Fig. 6

Frequency of CD4⁺CD25⁻FoxP3⁺ (a) and CD4⁺CD25⁺FoxP3⁺ T cells (b) in lymph node, spleen and TIL. Cells were stained with anti-mouse CD4 and anti-mouse CD25 and after permeabilization, cells were stained additionally with FoxP3. Data represent average of 3 independent experiments. Significance between control and experimental groups were determined by Student’s t-test