Table 1 Rat primers used for qRT-PCR analysis
Gene | Primer sequence |
|---|---|
TNMD | F: 5′-CCATGCTGGATGAGAGAGGTTAC-3′ R: 5′-CACAGACCCTGCGGCAGTA-3′ |
SCX | F: 5′-AACACGGCCTTCACTGCGCTG-3′ R: 5′-CAGTAGCACGTTGCCCAGGTG-3′ |
COL1a1 | F: 5′-CCGGACTGTGAGGTTAGGAT-3′ R: 5′-AACCCAAAGGACCCAAATAC-3′ |
COL3a1 | F: 5′-AACGGAGCTCCTGGCCCCAT-3′ R: 5′-ATTGCCTCGAGCACCTGCGG-3′ |
IL-1B | F: 5′-AGCAGCTTTCGACAGTGAGG-3′ R: 5′-CTCCACGGGCAAGACATAGG-3′ |
IL-6 | F: 5′-AGAAAAGAGTTGTGCAATGGCA-3′ R: 5′-GGCAAATTTCCTGGTTATATCC-3′ |
IFN-γ | F: 5′-AGGCCATCAGCAACAACATAAGTG-3′ R: 5′-GACAGCTTTGTGCTGGATCTGTG-3′ |
IL-10 | F: 5′-GGACTTTAAGGGTTACTTGGG-3′ R: 5′- AGAAATCGATGACAGCGTCG-3′ |
IL-4 | F: 5′-GAACTCACTGAGAAGCTGCA-3′ R: 5′-GAAGTGCAGGACTGCAAGT-3′ |
IL-13 | F: 5′-AGACCAGAAGACTTCCCTGT-3′ R: 5′-TCAATATCCTCTGGGTCCTG-3′ |
CCR7 | F: 5′-TGGTCATTTTCCAGGTGTGCT-3′ R: 5′-TACAGGGTGTAGTCCACGGT-3′ |
CD163 | F: 5′-GTAGTAGTCATTCAACCCTCAC-3′ R: 5′-CGGCTTACAGTTTCCTCAAG-3′ |