Fig. 4

SAH5-EJ1 effectively treats models of basal and inflammatory breast cancers while increasing survival. a Changes in tumor burden in breast cancer PDX mouse model TM01278 with various concentrations of intravenous SAH5-EJ1. Control represents water as a vehicle control. *p < 0.05 (Control vs 1 µg/g); **p < 0.01 (Control vs 1 µg/g and vs 10 µg/g). N = 6 for each condition. b Kaplan–Meier curve of breast PDX mouse model treated with control (blue) or either 1 or 10 µg/g SAH5-EJ1 (green and red, respectively) (red asterisks indicate time point at which mice could no longer receive injections due to site toxicity). Data shown represent mean. N = 6 for all groups. c Cell viability assay performed in SUM149 cells over 3 days comparing treatment with Sapitinib (blue) to SAH5-EJ1 (red) and SAH5-CP (grey). Solid lines represent 24 h; dashed lines represent 72 h. Data shown represent mean ± percent difference of assays performed in triplicate. d Kaplan–Meier curve of inflammatory breast cancer SUM149-injected mouse model treated with injected control (water) (blue) or either 0.5 or 5 µg/g SAH5-EJ1 (green and red, respectively). Data shown represent mean. N = 8 for all groups. e Changes in tumor burden in SUM149-generated tumors with various doses of SAH5-EJ1. Control represents water (blue). Arrow indicates transition from intravenous (IV) SAH5-EJ1 injections to intraperitoneal (IP) on day 66. **p < 0.01 (Control vs 0.5 µg/g and vs 5 µg/g). Data shown represent mean ± standard deviation. N = 8 for all groups. f Images of mouse tumors taken on Day 116. Arrowheads indicate tumor site. Representative images selected. N = 8 for each condition. g Lysates were taken from tumors in mice injected with SAH5-EJ1 intravenously (IV) for 31 days of the study or mice transitioned to intraperitoneal flank (IP) injections for the remainder of the study. Control lanes represented by untreated SUM149 cells. Lysates separated by SDS-PAGE and molecular weights are indicated on the right