Table 4 Primer sequences for reverse transcription quantitative polymerase chain reaction
Gene | Primer sequence |
|---|---|
miR-195-5p | Forward: 5′-CGGGATCCACATCTGGGGCCTTGTGA-3′ |
Reverse: 5′-CCCAAGCTTGCTTCGTGCTGTCTGCTT-3′ | |
PSAT1 | Forward: 5′-TGCCCAGAAGAATGTTGGCT-3′ |
Reverse: 5′-TCCAGAACCAAGCCCATGAC-3′ | |
β-actin | Forward: 5′-GATTCCTATGTGGGCGACGAG-3′ |
Reverse: 5′-CCATCTCTTGCTCGAAGTCC-3′ | |
U6 | Forward: 5′-CTCGCTTCGGCAGCACA-3′ |
Reverse: 5′-AACGCTTCACGAATTTGCGT-3′ |