Fig. 2
From: Reduced eIF3d accelerates HIV disease progression by attenuating CD8+ T cell function
The expression levels of eIF3d in CD8+ T cells from chronic HIV-infected patients were reduced, and eIF3d siRNA transfection suppressed CD8+ T cell function. a Expression of eIF3d in five types of sorted cells in peripheral blood from HCs (P = 0.026). b The CD8+ T cell sorting purity was 99.3%. c The expression levels of eIF3d mRNA in CD8+ T cells of HCs, treatment-naïve chronic HIV-infected patients and ART patients (P = 0.008, P = 0.013). d The eIF3d mRNA in the same patients after 2 years of ART was higher than it was before treatment (P = 0.018). e Expression of eIF3d in CD8+ T cells from treatment-naïve chronic HIV-infected patients was positively correlated with CD4+ T cell counts (P = 0.001). f EIF3d siRNA treatment reduced eIF3d mRNA levels in CD8+ T cells by 60% (P = 0.012). Representative flow cytometry plots and statistical charts showing that eIF3d siRNA transfection suppressed CD8+ T cell proliferation (g, h) and secretion of IFN-γ (k, l) and promoted CD8+ T cell apoptosis (i, j)