Fig. 1

Frequencies of Helios⁺ Tregs in MG patients compared to HDs. a The FMO and isotype controls are shown. b Analysis of CD25, FOXP3, and Helios expression among CD4⁺ T cells in representative peripheral blood samples from HDs and patients with MG. c Frequencies of Helios⁺ Tregs among CD4⁺ T cells in HDs (n = 14), and patients with OMG (n = 14), and GMG (n = 16) (median/interquartile range values). d Correlation between frequencies of Helios⁺ Tregs among CD4⁺ T cells in MG patients with their QMG scores. e, f Frequency of Tregs in GMG patients decreased compared to HDs (p = 0.0059) and there was no different of CD4⁺ T frequency in each groups (p = 0.1435) (*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001)