Table 3 Predicted and experimentally validated target genes of miR-24, miR-23a and miR-145 in TGFβ- and apoptosis-related pathways
Target | miRNA | ||
|---|---|---|---|
Gene symbol | Pathway | Prediction | Experiment |
ACVR1B | Role of activin A in cytoskeleton remodeling | miR-145 | miR-24 (2), miR-145 (6,11) |
ACVR2A | Role of activin A in cytoskeleton remodeling | miR-145 | |
APAF1 | FAS signaling cascades | miR-23a | miR-23a (17,20,23,25,28,34,42) |
CASP7 | FAS signaling cascades | miR-23a | miR-23a (7,12,21) |
CFL2 | TGF, WNT and cytoskeleton remodeling/TGFβ-dependent induction of EMT via rhoA and PI3 K | miR-23a | |
CHUK | TGF, WNT and cytoskeleton remodeling/TGFβ-dependent induction of EMT via rhoA and PI3K | miR-23a | |
COL4A1 | TGF, WNT and cytoskeleton remodeling | miR-23a | |
FAS | FAS signaling cascades | miR-23a | miR-23a (3,15,22) |
GSK3B | TGF, WNT and cytoskeleton remodeling/TGFβ-dependent induction of EMT via rhoA and PI3K | miR-23a | |
LRP5 | TGF, WNT and cytoskeleton remodeling | miR-23a | miR-23a (36), miR-145 (38) |
MAP3K1 | FAS signaling cascades | miR-23a | |
MAP3K5 | FAS signaling cascades | miR-23a | |
MAPK14 | TGFβ-mediated regulation of cell proliferation | miR-24 | miR-24 (1) |
MKL2 | TGFβ-dependent induction of EMT via SMADs | miR-145 | |
MYL12B | TGF, WNT and cytoskeleton remodeling | miR-23a | |
NLK | TGF, WNT and cytoskeleton remodeling | miR-23a | |
PAK2 | FAS signaling cascades | miR-23a | |
PDGFRA | TGFβ-mediated regulation of cell proliferation | miR-24 | |
PDGFRB | TGFβ-mediated regulation of cell proliferation | miR-24 | miR-24 (16,33) |
PDPK1 | TGFβ-dependent induction of EMT via rhoA and PI3 K | miR-23a | |
PIK3C2A | TGF, WNT and cytoskeleton remodeling/TGFβ-dependent induction of EMT via rhoA and PI3K | miR-23a | |
PIK3CB | TGF, WNT and cytoskeleton remodeling/TGFβ-dependent induction of EMT via rhoA and PI3K | miR-23a | |
PPP1CB | TGF, WNT and cytoskeleton remodeling | miR-23a | |
PPP1R12A | TGF, WNT and cytoskeleton remodeling | miR-23a | |
PXN | Role of activin A in cytoskeleton remodeling | miR-145 | miR-145 (30) |
SERPINE1/PAI1 | TGFβ-dependent induction of EMT via SMADs | miR-145 | miR-145 (8,13) |
SMAD2 | TGFβ-dependent induction of EMT via SMADs/role of activin A in cytoskeleton remodeling | miR-145 | miR-145 (5,40) |
SMAD3 | TGFβ-dependent induction of EMT via SMADs/role of activin A in cytoskeleton remodeling | miR-145 | miR-23a (39,41), miR-145 (14,27,29,31,37) |
TCF3 | TGFβ-dependent induction of EMT via SMADs | miR-145 | miR-24 (19) |
TGFBR2 | TGF, WNT and cytoskeleton remodeling/TGFβ-dependent induction of EMT via rhoA and PI3K/TGFβ-dependent induction of EMT via SMADs | miR-23a, miR-145 | miR-145 (18,26,40) |
TJP1 | TGFβ-dependent induction of EMT via rhoA and PI3K | miR-23a | |
XIAP | TGF, WNT and cytoskeleton remodeling/FAS signaling cascades | miR-23a | miR-23a (4,9,35), miR-24 (10,24,32) |