Table 1 Primer sequences for the real time PCR
From: Role of MAML1 in targeted therapy against the esophageal cancer stem cells
| Â | Sequence | Thermal profile | Size (bp) |
|---|---|---|---|
HES1 | F: CCCAACGCAGTGTCACCTTC R: TACAAAGGCGCAATCCAATATG | 95 °C(10 min)[95 °C(30 s)/58 °C(30 s)/72 °C(30 s)]40 | 304 |
GAPDH | F: GGAAGGTGAAGGTCGGAGTCA R: GTCATTGATGGCAACAATATCCACT | 95 °C(10 min)[95 °C(30 s)/58 °C(30 s)/72 °C(30 s)]40 | 108 |
HEY1 | F: ACGGCAGGAGGGAAAGGTTAC R: CTGGGAAGCGTAGTTGTTGAGATG | 95 °C(10 min)[95 °C(30 s)/58 °C(30 s)/72 °C(30 s)]40 | 294 |
HEY2 | F: AGAAAAGGAGAGGGATTATAGAGAAAAGG R: AGCGTGTGCGTCAAAGTAGC | 95 °C(10 min)[95 °C(30 s)/58 °C(30 s)/72 °C(30 s)]40 | 300 |
Nanog | F: GCAATGGTGTGACGCAGAAGGC R: GCTCCAGGTTGAATTGTTCCAGGTC | 95 °C(10 min)[95 °C(30 s)/65 °C(30 s)/72 °C(30 s)]40 | 137 |
Bmi1 | F: CGTGTATTGTTCGTTACCTGGAGAC R: CATTGGCAGCATCAGCAGAAGG | 95 °C(10 min)[95 °C(30 s)/62 °C(30 s)/72 °C(30 s)]40 | 204 |
CD44s | F: TCCAACACCTCCCAGTATGACA R: GGCAGGTCTGTGACTGATGTACA | 95 °C(10 min)[95 °C(15 s)/60 °C(60 s)]40 | 83 |
CD44v3 | F: GCACTTCAGGAGGTTACATC R: CTGAGGTGTCTGTCTCTTTC | 95 °C(10 min)[95 °C(15 s)/60 °C(60 s)]40 | 181 |
CD44v6 | F: AGGAACAGTGGTTTGGCAAC R: CGAATGGGAGTCTTCTCTGG | 95 °C(10 min)[95 °C(15 s)/60 °C(30 s)]40 | 68 |
MAML1 | F: TCTCGCGGAACAGGAGA R: GCAGCAGAGGACCCTGTG | 95 °C(10 min)[95 °C(30 s)/58 °C(30 s)/72 °C(30 s)]40 | 123 |