Fig. 3

Level of IL32 isoform expression after TNFα treatment. a IL32 gene expression (α, β, γ) after 3 day treatment with 1000 U/mL TNFα was compared to two IL32 expressing melanoma cell lines (M318 and M418) and Jurkat cells. Gene expression was normalized to GAPDH and expressed as Delta Ct values, with the Jurkat cells as the reference control. b Induction of IL32 gene expression with 24, 48, and 72 h treatment with 1000 U/mL TNFα. Gene expression was normalized to GAPDH and Delta Ct values are compared to the day 0 reference control