Fig. 8
Panel of representative histopathological findings in the different diet groups. 1st row: SD + FFC/SD normal hepatocytes without cytoplasmic alterations (CA) (CA score 0). FFC Hepatocytes with cytoplasmic alterations (CA score 3). FFCDIA Hepatocytes with cytoplasmic alterations (CA score 3) plus macrovesicular steatosis (steatosis score 1). Hematoxylin and eosin (HE) staining, magnification ×40. 2nd row: SD Normal collagen septa surrounding the lobules (fibrosis score 0). FFC Periportal and perisinusoidal fibrosis (fibrosis score 2). FFC/SD Portal/periportal fibrosis seen as excessive collagen deposition along septa and portal areas (fibrosis score 1C). FFCDIA Bridging fibrosis with collagen deposition from portal area to central vein (fibrosis score 3). Picro-sirius red (PSR) staining, magnification ×5. 3rd row: SD + FFC/SD normal background staining of macrophages. FFC + FFCDIA Increased staining of macrophages compared to normal. Immunochemical staining with antibodies against Iba1, magnification ×20. 4th row: Periodic-acid Schiff (PAS) staining for glycogen SD ‘+’ (PAS positive score 1). FFC ‘++’ (PAS positive score 2). FFC/SD ‘−’ (PAS positive score 0). FFCDIA ‘++’ (PAS positive score 2). PAS staining without pretreatment with diastasis, magnification ×20. 5th row: SD + FFC + FFC/SD + FFCDIA Pretreatment with diastasis for degradation of glycogen before staining with periodic-acid Schiff (PASd). Used as control for intensity of glycogen (PAS) staining without pretreatment, magnification ×20. SD Lean control group, FFC high fat/fructose/cholesterol diet group, FFC/SD diet-normalization group, FFCDIA diabetic group