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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Crosstalk between tumor cells and lymphocytes modulates heparanase expression

Fig. 4

Expression of heparanases and syndecan-1. a Flow cytometer analysis to detect heparanase isoforms (HPSE and HPSE2) in circulating T-lymphocytes. T-Lymphocytes, lymphocytes collected from a healthy woman; T-lymphocytes were activated during 4 h at 37 °C as indicated in the presence of, MCF-7 cells (+ MCF-7 cells); conditioned medium collected from co-culture (+ co-culture medium); plasma obtained from breast cancer patient (+ breast cancer plasma); plasma obtained from lung cancer patient (+ lung cancer plasma); plasma from gastric cancer patient (+ gastric cancer plasma); plasma from breast cancer patient that has bone metastasis (+ breast cancer and bone metastasis plasma). All values represent the median and standard deviation performed in triplicate assays. The analyses were performed using Kruskal–Wallis test with Dunn auxiliary test. *HPSE/HPSE2 expression compared to the expression of non-activated T-lymphocytes, *p < 0.0001; b mRNA expression of heparan sulfate proteoglycan syndecan-1 (SYN-1) in circulating T-lymphocytes obtained by qPCR analysis. The assays were performed using lymphocytes collected from five healthy donors and plasma samples collected from four different patients with diverse types of cancer. Therefore, the values represent median and range of the triplicate experiments. RT-PCR reaction was performed in quadruplicates for each independent sample. */**syndecan-1 expression compared to the expression obtained of non-activated T-lymphocytes, *p < 0.005 and **p < 0.0001

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