Fig. 5

Expression levels of IL-6 mRNA and protein in THP-1 cells transfected with miR-338-5p followed by 6 h of stimulation with LPS. a Taqman analysis of endogenous levels of IL-6 after LPS induction, showed that transfection with miR-338-5p reduced IL-6 transcripts compared to control miRNA (miR-ctrl). b Overexpression of miR-338-5p decreased the secreted IL-6 content. ELISA using cell culture supernatants of THP-1 cells stimulated with LPS showed lower levels of the proinflammatory mediator secreted by miR-338-5p transfected cells compared to control miRNA transfection. Significance was determined by one-way ANOVA with Dunnet’s post hoc test, ***p < 0.001