Fig. 4

Luciferase assay to determine miR-338-5p binding to the 3′UTR of predicted mRNA targets. a The miR-338-5p targeting sequence in the 3′-untranslated region of human IL6 mRNA. b Plasmids with the 3′UTR of IL-6 cloned downstream of the luciferase open-reading frame were cotransfected with miR-338-5p, anti-miR-338 or control miRNA mimic in HEK293 cells. Luciferase activity was measured 48 h after transfection, and the data expressed as percentage of luminescence. Firefly luciferase measurements were normalized to Renilla and the average of three independent experiments is shown. Statistically significant difference from control was calculated using Student t-test, *p < 0.05