Fig. 6

Expression of hsa_circ_0055538 affects the levels of key proteins involved in the p53/Bcl-2/caspase signaling pathway. a, b Wound healing assays were performed using a SCC9 and b CAL27 cells overexpressing p53 after knockdown of hsa_circ_0055538. The scratch area was measured at 0 and 24 h, and the percentage of closure at 24 h was calculated. c Invasion assays were performed using SCC9 (top) and CAL27 (bottom) cells overexpressing p53 after knockdown of hsa_circ_0055538. Cells were seeded into the Matrigel-coated upper chamber; after 48 h, those that passed across the coated chamber were imaged and quantified. The proportion of invading cells was quantified in SCC9 (left) and CAL27 (right) cells. d SCC9 and CAL27 cells were subjected to either the overexpression (left) or knockdown (right) of hsa_circ_0055538. The mRNA level of the RMND5A was detected by qRT-PCR. Data are presented as mean ± SEM of three independent experiments. Student’s t-test, ***P < 0.001, **P < 0.01