Fig. 7

Toxicity screen of Sylatron and Actimmune on primary human cells. a Myofibroblasts, b renal epithelial cells, c human vascular endothelial cells (HUVEC), d primary hepatocytes were assayed with Sylatron (light green bars), Actimmune (light blue bars), Sylatron and Actimmune (light red bars) or Sylatron and monocytes (dark green bars), Actimmune and monocytes (dark blue bars), Sylatron, Actimmune, and monocytes (dark red bars) for 3 days and cell viability was measured using trypan blue. Staurosporine (black bars) was used as a positive control for cell toxicity. Experiments were performed three times with three healthy donor monocytes, except for the hepatocytes which were repeated three times with three separate primary hepatocyte donors. Yellow asterisks indicate comparison to cell control; purple indicate comparison to monocyte-only control. Numbers of asterisks indicate p-value as follows: * < 0.05; ** < 0.01; *** < 0.001; **** < 0.0001