Fig. 4

Workflow for elutriated fraction RO for the quantification of percent monocytes. a Cells from the final product from a healthy donor after the addition of interferons were gated on singlets, followed by CD45⁺ cells, live cells, and then the CD15⁻Lin⁻ population. Total monocytes were counted as the combination of Classical (CD14⁺), intermediate (CD14⁺CD16⁺), and non-classical CD14⁻/^dimCD16⁺. b Shows the backgating of the monocyte population. c Analysis of the same product from a after 4 h incubation at room temperature. d Shows the backgating strategy as in b