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Table 1 Primer oligonucleotide sequences (forward and reverse) used in RT-qPCR

From: Dermal tissue remodeling and non-osmotic sodium storage in kidney patients

Primer Forward sequence Reverse sequence
A. Inflammation
 CCL2 5′-AGACTAACCCAGAAACATCC-3′ 5′-ATTGATTGCATCTGGCTG-3′
 VCAM 5′-TCCTGAGCTTCTCGTGCTCTATT-3′ 5′-TGACCCCTTCATGTTGGCTT-3′
B. Fibrosis
 COL1A1 5′-GGGATTCCCTGGACCTAAAG-3′ 5′-GGAACACCTCGCTCTCCA-3′
C. Lymphangiogenesis
 VEGFC 5′-CTGGCTCAGGAAGATTTTATG-3′ 5′-TGTTTTTACAGACACACTGG-3′
 PDPN 5′-AAGATGGTTTGTCAACAGTG-3′ 5′-GTACCTTCCCGACATTTTTC-3′
D. Proteoglycan related
 VCAN 5′-CCAGTGTGAACTTGATTTTG-3′ 5′-CAACATAACTTGGAAGGCAG-3′
 NDST1 5′-CGTGACGCGACCTAGCGA-3′ 5′-TCATAGGTGGAGTGATTTGACTGG-3′
 HS6ST1 5′-AGGAAGTTCTACTAACATCACC-3 5′-CCCATCACACATATGCAAC-3′
 HSPE 5′-CCTTGCTATCCGACACCTTT-3′ 5′-GGCTGACAGGCCCAATTTA -3′
 CHYSY1 5′-AGACTTTCAGCAAAATCCAG-3′ 5′-GTTTGAGAGAAAGGACAAGG-3′
 HAS1 5′-TCCACTGTGTATCCTGCATC-3′ 5′-CCCCAAAAGTATCCTGCATC -3′
 HAS2 5′-GATGCATTGTGAGAGGTTTC-3′ 5′-CCGTTTGGATAAACTGGTAG-3′
 HAS3 5′-CTTGAAGATTAATGTAGGATGACAGGCT-3′ 5′-AAAGTTGACGACCACAGTGCAA-3′
 UST 5′-GAACGTGAATGAAAACTTCC-3′ 5′-TCTGGGTCTTTGTAGATACTG-3′
 CHST11 5′-TATTTCCAAATCATGCGGAG-3′ 5′-ATTGGGTTGTAGAGTTCCTG-3′
E. Housekeeping
 β-Actin 5′-CCAACCGCGAGAAGATGA-3′ 5′-CCAGAGGCGTACAGGGATAG-3′