Fig. 1

Expression of total PFKFB2 and phosphorylation of PFKFB2 at Ser483. Western blots obtained by immunoblotting antibodies directed against total PFKFB2, Ser483 phosphorylated PFKFB2 and CD9 protein (a, c). Densitometry analysis shows a 4.7-fold increase in PFKFB2 expression in the PE group as compared to the NP group, represented as a ratio of PFKFB2 to CD9 expression (b). d Shows the 2.6-fold increase in PFKFB2 phosphorylation at the Ser483 residue. Data is represented as scatter plots, with each individual patient densitometry value represented as a dot, with the horizontal line representing the median. e Western blot demonstrating proportional presence of the exosomal markers CD9, TSG101 and ALIX in the prepared samples