Fig. 4

Effect of adiponectin on primary cells. Effect of adiponectin on human primary osteoblast. a, b Primary osteoblast were exposed to 1–10 μg/mL adiponectin for 48 h. RANKL and OPG mRNA expression were determined by quantitative real-time PCR. The Y axis represents the fold change and the expression of RANKL and OPG at 0 μg/mL were set to 1.0. Effect of adiponectin on human primary chondrocyte. c Primary chondrocyte were exposed to 0.1–1 μg/mL adiponectin for 24 h. IL6 mRNA expression were determined by quantitative real-time PCR. The Y axis represents the fold change and the expression of IL6 at 0 μg/mL were set to 1.0. One way ANOVA was use to tested, when p value > 0.05, there was no significant difference in all the groups; when p value < 0.05, then using bonferroni correction to test each two groups. *p < 0.0167 Bonferroni correction test between controls and AIS concave. Bonferroni correction test between controls and AIS convex. ^#p < 0.0167 Bonferroni correction test between AIS concave and AIS convex