High throughput sequencing of T-cell receptor repertoire using dry blood spots

Table 1 The clinical characteristics of samples with low, moderate and high TCR repertoire diversities

Diversity	Low		Moderate		High
Sample	WB	DBS	WB	DBS	WB	DBS
Duplicate sequence repeatability (R²)	0.994	0.991	0.993	0.956	0.930	0.699
Unique CDR3	43,653	5327	38,431	3820	25,118	3208
D50	0.3	1.1	2.7	7.6	14.5	6.8
Diversity Index	15.8	13.2	20.6	16.8	27.1	11.1
R² of regression between DBS and WB		0.989		0.865		0.806
Top 50 dominant clones (%)^a		94%		98%		82%
Top 100 dominant clones (%)^a		92%		87%		74%

WB whole blood, DBS dry blood spot, R² coefficient of determination calculated by linear regression
^aThe percentage of TCR dominant clones from whole blood could be found from DSB examination

ISSN: 1479-5876