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Table 1 The clinical characteristics of samples with low, moderate and high TCR repertoire diversities

From: High throughput sequencing of T-cell receptor repertoire using dry blood spots

Diversity Low Moderate High
Sample WB DBS WB DBS WB DBS
Duplicate sequence repeatability (R2) 0.994 0.991 0.993 0.956 0.930 0.699
Unique CDR3 43,653 5327 38,431 3820 25,118 3208
D50 0.3 1.1 2.7 7.6 14.5 6.8
Diversity Index 15.8 13.2 20.6 16.8 27.1 11.1
R2 of regression between DBS and WB   0.989   0.865   0.806
Top 50 dominant clones (%)a   94%   98%   82%
Top 100 dominant clones (%)a   92%   87%   74%
  1. WB whole blood, DBS dry blood spot, R2 coefficient of determination calculated by linear regression
  2. aThe percentage of TCR dominant clones from whole blood could be found from DSB examination