Fig. 7
Detection of INF-γ producing CD8+ T. Cellular response was assay by ELIspot counting the number of INF-γ spots forming units after ex vivo stimulation of splenocytes with DBP418–426 peptide a, c EXP.1, b, d EXP.2. Group means are represented as in the histograms. c, d Exemplary samples. The nonspecific activator anti-CD3 antibody was used as positive control for the assay. (EXP.1 and EXP.2 n = 3 mice/group. Data are analyzed by multiple t-test of splenocytes from control and treated mice stimulated with media, spontaneous, or DBP418–426, stimulated, *p < 0.05, ***p < 0.001)