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Table 3 Quantitative real-time polymerase chain reaction (qPCR) and mRNA microarray analysis for selected mRNAs

From: MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo

mRNA qPCR miRNA microarray
Fold change P-value Regulation Fold change P-value Regulation
POD 5
 HIF1A 2.18 0.214 Up 1.97 0.0003 Up
 TLR6 4.00 0.071 Up 4.11 0.0003 Up
 VEGFA 3.45 0.22 Up 4.41 0.0007 Up
 THBS3 − 14.29 0.001 Down − 8.78 5.33 × 10−6 Down
 NDRG2 − 33.33 0.002 Down − 21.00 0.0005 Down
POD 10
 HIF1A 1.69 0.049 Up 1.59 0.0380 Up
 TLR6 2.72 0.061 Up 2.22 0.1089 Up
 VEGFA 3.46 0.015 Up 3.12 0.0379 Up
 THBS3 − 7.14 0.001 Down − 1.36 0.0050 Down
 NDRG2 − 7.69 0.003 Down − 10.91 0.0157 Down
POD 15
 HIF1A 1.19 0.288 Up 1.38 0.3585 Up
 TLR6 2.22 0.003 Up 2.46 0.0106 Up
 VEGFA 2.32 0.066 Up 2.71 0.1847 Up
 THBS3 − 11.11 0.001 Down − 1.57 0.0008 Down
 NDRG2 − 11.11 0.003 Down − 8.48 0.0002 Down
  1. POD postoperative day, HIF1A hypoxia-inducible factor-1 A, TLR 6 toll-like receptor 6, VEGFA vascular endothelial growth factor-A, THBS3 thrombospondin 3, NDRG2 NMYC downstream-regulated gene 2, POD postoperative day