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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: The important role of connexin 43 in subarachnoid hemorrhage-induced cerebral vasospasm

Fig. 4

Pretreatment of Cx43-targeting siRNA leads to knockdown of BA Cx43 expression and PKC inhibitors reversed the increased Cx43 on day 7 after SAH. a After cisterna magna injection with either non-targeting siRNA (control) or Cx43-targeting siRNA, expression levels of Cx43 in BAs were assessed by Western blotting over time (days). The upper photographs are from a representative immunoblot and quantitative graphs are shown below. **P<0.01 vs. rat injected with control siRNA at the corresponding time point, n = 8. Bars indicate the mean ± SEM. The uncropped blots are presented in Additional file 1: Figs. S5, S6. b The effect of PKC inhibitors on Cx43 protein level on day 7 after SAH. Quantitative graphs are shown below, data represent the mean ± SEM, **P<0.01 vs. normal group, ##P<0.01 vs. OxyHb-only group, n = 8. c Localization for DAPI and Cx43 in rat subjected to surgery. Tissues were taken 7 days after SAH in each group. From top to bottom the groups are: SAH + control siRNA, SAH + Cx43 siRNA, SAH + CHE and SAH + GF, respectively. Scale bar = 15 μm

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