Fig. 3

ROR2 overexpression induced cell apoptosis of HGSCO cells. a HEY, OV-90 and HO-8910 cells were transfected with ROR2 overexpression or negative control adenovirus for 72 h. Apoptosis was detected by flow cytometry after staining with FITC Annexin-V and PI. b Quantitive analysis of apoptotic ratio in HEY, OV-90 and HO-8910 cells with CellQuest Pro software. Statistical analysis was performed with GraphPad Prism using Student’s t test. c HEY, OV-90 and HO-8910 cells were transfected with ROR2 overexpression or negative control adenovirus for 72 h. Expression of ROR2, Bcl-2, Bax, caspase3, cleaved caspase3, caspase7, cleaved caspase7, PARP and cleaved PARP were determined by western-blot assay. β‑actin was used as a loading control. d Quantitation of wester-blot assay bands shown in c using Image J. Statistical analysis was performed using Student’s t test. NC: negative control. All experiments were repeated three times at least. *P < 0.05, **P < 0.01 and ***P < 0.001 for statistical analysis of the indicated groups