Fig. 2

ROR2 overexpression inhibited proliferation and colony formation of HGSCO cells. a Western-blot assay was used to detect the expression of ROR2 in HEY, A2780, OV-90 and HO-8910 cells. b Western-blot assay was used to detect the protein expression of ROR2 in HEY, OV-90 and HO-8910 cells after being transfected with ROR2 overexpression or negative control adenovirus for 72 h. c PCR assay was used to detect the mRNA expression of ROR2 in HEY, OV-90 and HO-8910 cells after transfected with ROR2 overexpression or negative control adenovirus for 48 h. d HEY, OV-90 and HO-8910 cells were transfected with ROR2 overexpression or negative control adenovirus, and proliferation ability of the transfected HGSOC cells was detected at a fixed time for 6 days by MTT assay. The growth curves were analyzed using 2way ANOVA test. e HEY, OV-90 and HO-8910 cells were transfected with ROR2 overexpression or negative control adenovirus for 24 h before being planted into six-well plates for colony formation assay. The colony formations were harvested after 12 days. f Colony formations were counted and analyzed with Student’s t test. NC negative control. All experiments were repeated three times at least. *P < 0.05, **P < 0.01 and ***P < 0.001 for statistical analysis of the indicated groups