Fig. 7
Metabolic cage study of animals with plasmid injection of pLIVE-GDF11 or pLIVE-SEAP. Mice were fed an HFD for 8 weeks after gene transfer and individually caged. Oxygen consumption and CO2 release by each mouse were monitored. The dark and light phases were indicated by black and white bars on the x-axis. a Oxygen consumption; b Average oxygen consumption in day and night; c CO2 output; d average CO2 output in day and night; e calculated energy expenditure; f average energy expenditure in day and night; g respiratory exchange ratio (RER); h accumulated activity; i the average rectal temperature after treatment. The rectal temperature of mice was measured weekly after hydrodynamic gene delivery. Data represent mean ± SEM (n = 5), #P < 0.05