Fig. 2

a, b Measurement of cytokines production following vaccinations. Splenocytes from vaccinated mice and controls (5 animals/group) were collected post-mortem and analyzed by ELI-Spot assay. Data are presented as the frequency of IFN-γ and TNF-α spot-forming cells per 106 splenocytes. Spot numbers represent the mean of ten mice for each vaccination; bars, SD calculated in triplicates. Two-tailed T-test p value versus no treatment group < 0.05 (*) or < 0.01 (**)