Fig. 4

BZRAP1-AS1 regulates the development of HCC by negatively regulating THBS1 via DNMT3b. a Transfection efficiency of sh-DNMT3b assessed by RT-qPCR. b Transfection efficiency of oe-THBS1 assessed by RT-qPCR. c Effects of BZRAP1-AS1, DNMT3b and THBS1 on the proliferation of HUVECs evaluated by EdU assay (×200). d Effects of BZRAP1-AS1, DNMT3b and THBS1 on the HUVEC migration evaluated by Transwell assay (×40). e Effects of BZRAP1-AS1, DNMT3b and THBS1 on angiogenesis evaluated by tube formation assay (×50). f The level of VEGFA in HuH-7 concentrated supernatant analyzed by ELISA. g Effects of BZRAP1-AS1, DNMT3b and THBS1 on angiogenesis detected by CAM assay (×20). *p < 0.05 vs. cells infected with lentivirus expressing sh-NC; ^#p < 0.05 vs. cells infected with lentivirus expressing oe-NC; ^&p < 0.05 vs. cells infected with lentivirus expressing oe-BZRAP1-AS1. The data (mean ± S.D.) obeying the normal distribution and homogeneity of variance between two groups were analyzed using unpaired t test while those among multiple groups were assessed by one-way ANOVA, followed with Tukey’s post hoc test. The experiment was repeated 3 times independently. BZRAP1-AS1 BZRAP1-antisense RNA 1, DNMT DNA methyltransferase, HCC hepatocellular carcinoma, THBS1 thrombospondin1, RT-qPCR reverse transcription quantitative polymerase chain reaction, DNMT3b DNA methyltransferase 3B, EdU 5-ethynyl-2′-deoxyuridine, VEGFA vascular endothelial growth factor, ELISA enzyme-linked immunosorbent assay, CAM chicken chorioallantoic membrane, ANOVA analysis of variance, NC negative control