Fig. 2
BZRAP1-AS1 silencing suppresses HUVEC proliferation and angiogenesis. a The expression of BZRAP1-AS1 in HuH7 cells infected with lentivirus expressing sh-NC or sh-BZRAP1-AS1 determined by RT-qPCR. b The effect of BZRAP1-AS1 on the proliferation of HUVECs co-cultured with HuH7 cells revealed by EdU assay (×200). c The effect of BZRAP1-AS1 on the migration of HUVECs evaluated by Transwell assay (×40). d The effect of BZRAP1-AS1 on angiogenesis of HCC cells assessed by tube formation assay (×50). e The level of VEGFA in HuH-7 concentrated supernatant measured by ELISA. f The effect of BZRAP1-AS1 on angiogenesis evaluated by CAM assay (×20). *p < 0.05 vs. HuH-7 cells infected with lentivirus expressing sh-NC. The data (mean ± S.D.) obeying the normal distribution and homogeneity of variance among multiple groups were analyzed using one-way ANOVA, followed with Tukey’s post hoc test. The experiment was repeated 3 times independently. HCC hepatocellular carcinoma, BZRAP1-AS1 BZRAP1-antisense RNA 1, RT-qPCR reverse transcription quantitative polymerase chain reaction, EdU 5-ethynyl-2′-deoxyuridine, VEGFA vascular endothelial growth factor, ELISA enzyme-linked immunosorbent assay, CAM chicken chorioallantoic membrane, ANOVA analysis of variance, NC negative control, HUVECs human umbilical vein endothelial cells