Fig. 6

Loss of AGXT expression promoted the malignant phenotypes of HCC cell in vitro. a The mRNA expressions of AGXT in a panel of HCC cell lines were detected by quantitative real-time PCR. b Proliferation ability of HCC cells was measured by cell counting. c Transwell migration assay for HCC cells. d The transfection efficiency of siRNA was measured 48 h after Cy3-siTC transfection (original magnification, ×400); and the knockdown efficiency of siAGXT in Huh7 cells was determined by quantitative real-time PCR and western blot. e Proliferation of siAGXT transfected Huh7 cells was measured by CCK8 assay. f Transwell migration assay for siAGXT transfected Huh7 cells, representative images and quantitative results were provided (original magnification, ×200). g Cell cycle assay for siAGXT transfected Huh7 cells, representative histogram of the gated cells in G0/G1, S, and G2/M phases and quantitative analyses, at least 10,000 cells per sample. h Apoptosis assay, representative histogram and quantitative analyses of the cells in each phase, at least 10,000 cells per sample. Each bar represents mean ± SD of the data obtained from three independent experiments. ***P < 0.0001, **P < 0.001, *P < 0.05