Table 1 Primers for qRT-PCR
From: Atheroprotective effects of methotrexate via the inhibition of YAP/TAZ under disturbed flow
Gene | Forward | Reverse |
|---|---|---|
AMPK-α | TGATGTTGTAGTGACACCATTTAC | GAAGATGAGGGAAAGAATTAAGGG |
CTGF | ACCGACTGGAAGACACGTTTG | CCAGGTCAGCTTCGCAAGG |
CYR-61 | TGAAGCGGCTCCCTGTTTT | CGGGTTTCTTTCACAAGGCG |
VCAM1 | CAGTAAGGCAGGCTGTAAAAGA | TGGAGCTGGTAGACCCTCG |
ICAM1 | TTGGGCATAGAGACCCCGTT | GCACATTGCTCAGTTCATACACC |
IL-6 | CTCAATATTAGAGTCTCAACCCCCA | GAGAAGGCAACTGGACCGAA |
IL-8 | CCACCGGAGCACTCCATAAG | GATGGTTCCTTCCGGTGGTT |
YAP | GCTACAGTGTCCCTCGAACC | CCGGTGCATGTGTCTCCTTA |
TAZ | ATCCCCAACAGACCCGTTTC | GAACGCAGGCTTGCAGAAAA |
GAPDH | ACGGATTTGGTCGTATTGGGC | TTGACGGTGCCATGGAATTTG |