Fig. 4
From: Atheroprotective effects of methotrexate via the inhibition of YAP/TAZ under disturbed flow
Silencing YAP reduces adhesion molecule expression but does not alter p-AMPK expression under DF. a HUVECs were transfected with siYAP or siNC, subjected to DF for 24 h, and evaluated by western blotting to detect YAP, VCAM1, ICAM1, p-AMPK, and AMPK. *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. DF. b YAP, as measured by qRT-PCR and western blotting, in HUVECs after transfection with siNC or siAMPKα for 36 h. All data are shown as mean ± SD of triplicate experiments and are representative of three independent experiments. **p < 0.01, ****p < 0.0001 vs. siNC