Table 1 Primers for RHD gene amplification and sequencing

E1-s(= E1-seq)

TCCATAGAGAGGCCAGCACAA

D

AJ252314

5′UTR − 152 to − 132

E1-a

GCTATTTGCTCCTGTGACCACTT

D

Z97363

+40 to +18

E2-s

TGACGAGTGAAACTCTATCTCGAT

D

U66341

-1060 to -1037

E2-a

GGCATGTCTATTTCTCTCTGTCTAAT

D/CE

U66341, AB035189

+355 to +330

E2-seq

CCTGGATTCCTTGTGATACACG

D/CE

U66341, U66340

+227 to +206

E3-s

GTCGTCCTGGCTCTCCCTCTCT

D

AB035190

− 29 to − 8

E3-a

CTTTTCTCCCAGGTCCCTCCT

D/CE

AB035192, AB035191

+39 to +19

E3-seq

GGTCCCTCCTCCCAGCAC

D/CE

AB035192, AB035191

+28 to +11

E4-s

GCCGACACTCACTGCTCTTAC

D/CE

U77079, U77078

− 36 to − 16

E4-a

TGAACCTGCTCTGTGAAGTGC

D

Y10605

+194 to +174

E4-seq

GGGAGATTTTTTCAGCCAG

D/CE

Y10605, Y10604

+82 to +64

E5-s

TACCTTTGAATTAAGCACTTCACAG

D

Y10605

− 267 to − 243

E5-a

TTATTGGCTACTTGGTGCC

D/CE

Z97334, AB035197

+1024 to +1006

E5-seq

AGACCTTTGGAGCAGGAGTG

D/CE

Y10605, Y10604

− 53 to − 34

E6-s(= E6-seq)

CAGGGTTGCCTTGTTCCCA

D/CE

Z97334, Z97333

− 95 to − 97

E6-a

CTTCAGCCAAAGCAGAGGAGG

D

Z97334

+41 to +21

E7-s

TGCCCATCCCCCTTTGGTGGCC

D

Z97334

− 106 to − 85

E7-a

CCAAGGTAGGGGCTGGACAG

D

AB035194

+171 to +152

E7-seq

GTCTCACCTGCCAATCTGCT

D/CE

Z97334, Z97333

− 41 to − 22

E8-s

GGTCAGGAGTTCGAGATCAC

D

AB035194

− 593 to − 574

E8-a(= E8-seq)

GATGGGGCACATAGACATCC

D/CE

AB035196

+97 to +78

E9-s(= E9-seq)

GGTCCAGGAATGACAGGGCT

D

AB035196

− 162 to − 143

E9-a

CGCTGAGGACTGCAGATAGG

D

AB035185

+294 to +275

E10-s

CAAGAGATCAAGCCAAAATCAGT

D/CE

AB035185, AB035184

− 67 to − 45

E10-a

AGCTTACTGGATGACCACCA

D

X63097

+290 to +271

E10-seq

CAGTCTGTTGTTTACCAGATGTTGTTAT

D

X63097

3′UTR +261 to +234