|
Groups
|
Number of volunteers
|
Collecting volume
|
Volume for filtration
|
Volume for detection
|
|---|
|
Types of carrier materials
|
9
|
21 ml
|
20 ml
|
1 ml
|
|
Volume ratio of bone marrow/carrier material
|
3
|
29 ml
|
4/10/14 ml
|
1 ml
|
|
Filtration frequency
|
5
|
21 ml
|
20 ml
|
1 ml
|
|
Components and safety evaluation
|
6
|
22 or 24 ml
|
20 ml
|
1 + 1 + 2 mla
|
- a1 ml for counting of bone marrow nucleated cells and MSCs (six volunteers); 1 ml for primary bone marrow MSCs culture (six volunteers); 2 ml for bone marrow serum extraction to detecting soluble protein in bone marrow (one volunteer)
