Comparison and characterization of enriched mesenchymal stem cells obtained by the repeated filtration of autologous bone marrow through porous biomaterials

Table 1 Distribution of bone marrow aspiration

Groups	Number of volunteers	Collecting volume	Volume for filtration	Volume for detection
Types of carrier materials	9	21 ml	20 ml	1 ml
Volume ratio of bone marrow/carrier material	3	29 ml	4/10/14 ml	1 ml
Filtration frequency	5	21 ml	20 ml	1 ml
Components and safety evaluation	6	22 or 24 ml	20 ml	1 + 1 + 2 ml^a

^a1 ml for counting of bone marrow nucleated cells and MSCs (six volunteers); 1 ml for primary bone marrow MSCs culture (six volunteers); 2 ml for bone marrow serum extraction to detecting soluble protein in bone marrow (one volunteer)

ISSN: 1479-5876