Fig. 4
From: PRAME promotes epithelial-to-mesenchymal transition in triple negative breast cancer
PRAME overexpression mediates invasion through 3D Matrigel. a Inverted Matrigel invasion of TNBC cells stably transduced with control vector or full-length PRAME. Live cells were visualized using Calcein-AM and serial optical sections were acquired at 15 μm intervals, with increasing depth from left to right. Rate of invasion beyond 30 μm, indicated by red line, was quantified in 3 fields of view/biological replicate (n = 3). **p < 0.01. b Matrigel Boyden Chamber invasion assay of TNBC cells stably transduced with control vector or full-length PRAME, performed in QCM ECMatrix Cell Invasion Assay. *p < 0.05, n = 3 biological replicates