Fig. 6
DEX mediates tumour angiogenesis via VEGF. a Immunohistochemistry staining of mouse pulmonary metastatic sections with CD31 antibody. Brown lines indicate tumour blood vessels. b Tumour blood vessels were counted in ten randomly selected fields (six mice per group). c Expression of VEGF in CD11b+Ly6ClowLy6G+ (G-MDSC) and CD11b+Ly6ChighLy6G− (M-MDSC) cells isolated from lungs of mice was analyzed by RT-qPCR. Representative results of RT-qPCR from G-MDSC and M-MDSC isolated from lungs of mice on day 1 after surgery were shown. d, e G-MDSC and M-MDSC were isolated from lungs of mice on days 0, 1, 3 and 7 after surgery and the relative mRNA expression of VEGF was analyzed by RT-qPCR (three mice per group at each point). Data presented are representative of three replicated experiments. *P < 0.001. ru, relative units