Fig. 3

Disturbed flow decreases GCX expression and thickness. En face Z-projections and orthogonal views are shown for cells stained for WGA after exposure to a static, b DF, and c UF conditions. Cells were also stained with an antibody to tag HS in f static, g DF, and h UF conditions. a–h Dotted lines indicate the position of orthogonal views, and the scale bar = 30 μm. d, e Normalized WGA MFI (d), as an indicator of RFPEC coverage, and WGA thickness (e), determined as shown in Additional file 3: Fig. S3. i, j Similarly shows normalized HS (i) MFI/coverage and (j) thickness (also determined as shown in Additional file 3: Fig. S3). d–j Number of independent experiments “N” are shown for each condition, and *P < 0.05, **P < 0.01, and ***P < 0.001 are indicated