Fig. 6

Assessment of collagen fiber maturity within healing defects. a Representative polarized picro sirius red stained healing defects with ACS (top) and ACS/Talymed (bottom). BMP2 dose increases from left to right. 10× magnification. b Quantification of thin (green) immature, intermediate (yellow), and thick (red) mature collagen fibers correlates with total collagen quantification with ACS presenting more of all fiber types as compared to ACS/Talymed. Note that the clinical dose of BMP2 presents the least of all fiber types while the other BMP2 doses present robust populations of fibers of all maturity levels indicating active turnover and remodeling. n = 3 sections 40 µm apart from at least four individuals per group *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001