Fig. 3

Platelet spreading and clot retraction. a After PD treatment, washed human platelets were placed on fibrinogen-coated glass coverslips and allowed to spread at 37 °C for 90 min followed by staining with Alexa Fluor-546-labelled phalloidin and subsequent observation under a fluorescent microscopy (mean ± SD, n = 3). b PD-treated platelets were supplemented with 2 mM Ca²⁺ and 0.5 mg/ml fibrinogen and clot retraction was induced by thrombin (1 U/ml) treatment at 37 °C. Images were captured every 30 min (mean ± SD, n = 3). Compared with 0, **P < 0.01; ***P < 0.001