Skip to main content

Table 10 miRNA studies in other kidney diseases

From: Genomic approaches in the search for molecular biomarkers in chronic kidney disease

PMID Author Year Sample Patients Ethnics Methodology Main findings
28667184 Cárdenas-González 2017 Urine Discovery cohort:
10 LN
10 HC
Confirmation stage:
89 LN
119 HC
Replication stage:
86 LN
37 SLE
30 HC
Caucasian qRT-PCR
miRNA in situ hybridisation
2402 urinary microRNAs
Downregulation of miR-3201 and miR-1273e (> threefold; p < 0.0001) in LN patients
Association of miR-3201 and miR-1273e expression with endocapillary glomerular inflammation (p < 0.01)
28380212 Domenico 2017 Urine 41 graft dysfunction
8 stable patients
Brazil qRT-PCR: miR-142-3p Overexpression of urinary miR-142-3p in the acute tubular necrosis group (p < 0.001) and acute rejection group (p < 0.005) in comparison with stable patients
Trend to higher urinary miR-142-3p expression, in the acute tubular necrosis group, compared with the acute rejection group (p = 0.079)
ROC analysis: AUC was 0.77 (95%CI 0.62–0.92; p = 0.010). The cut-off selected was 2.03 and the resulting parameters were sensitivity = 92%; specificity = 87%; positive predictive value = 90% and a negative predictive value = 87% (p < 0.001)
Blood Overexpression of urinary miR-142-3p when acute tubular necrosis was compared with stable patients (p < 0.05) and with the acute rejection group (p < 0.05)
No difference was found in the comparison between the stable patients and acute rejection groups (p = 0.921)
ROC analysis: AUC was 0.75 (95%CI 0.56–0.94; p = 0.016). The cut-off selected was 1.59 and the resulting parameters were sensitivity = 75%; specificity = 63%; positive predictive value = 81%, and a negative predictive value = 50% (p < 0.05)
24489795 Ben-Dov 2014 Urine 20 CKD
20 ADPKD
  barcoded adapter ligation,
Illumina Genome Analyzer II
mir-143(2) members were 2.9-fold higher in urine cells from ADPKD compared to other CKD patients
Expression levels of mir-133b(2) (4.9-fold) and mir-1(4) (4.4-fold) were lower in ADPKD
25197634 Hu Yang-Yang 2014 Serum 60 Nephrolithiasis, 
50 HC
Asian qRT-PCR:
miR-155
The median levels of serum and urinary levels of miR-155 were significantly higher in nephrolithiasis patients than in controls
eGFR inversely correlated with urinary level of miR-155
22685016 Guan 2012 Urine 12 SLE
10 NecGN
  qRT-PCR The degree of proteinuria significantly correlated with the urinary sediment level of miR-339-3P (r = 0.636; p < 0.001), but not other miRNA targets
Urinary sediment miR-221 level correlated with serum C3 level (r = 0.658; p = 0.02).
In the SLE group, urinary sediment miRNA levels did not correlate with the histological activity or chronicity index of the renal biopsy specimen
21987229 Wang 2012 Urine 40 SLE
13 HC
Asian qRT-PCR: miR-146a and miR-155 Overexpression of urine miR-146a (2.27 [1.20–5.52] versus 0.87 [0.54–1.18]; p = 0.001) and miR-155 (2.28 [1.16–5.13] versus 0.99 [0.84–1.67]; p = 0.001) in SLE
Correlation of eGFR with the level of urinary miR-146a (r = 0.242; p = 0.008), but not miR-155 (r = 0.069; p = 0.456)
20952466 Wang 2010   40 SLE
30 HC
Asian qRT-PCR: miR-146a and miR-155 Downregulation of serum miR-146a and miR-155 levels, and upregulation of urinary level of miR-146a in SLE
eGFR correlated with both serum miR-146a (r = 0.519; p = 0.001) and miR-155 (r = 0.384; p = 0.014)
Serum miR-146a inversely correlated with proteinuria (r = − 0.341; p = 0.031) and the SLE Disease Activity Index (r = − 0.465; p = 0.003)
  1. ADPKD: autosomal dominant polycystic kidney disease; C3: complement component 3; CKD: chronic kidney disease; eGFR: estimated glomerular filtration rate; HC: healthy controls; NecGN: pauci-immune necrotizing glomerulonephritis; LN: lupus nephritis; qRT-PCR: quantitative real-time polymerase chain reaction; RNA: ribonucleic acid; ROC: receiver operating characteristic; SLE: systemic lupus erythematous