Fig. 3

Experimental binding of TPNAs to HLA-A*0201. Binding to HLA-A*0201 molecule and relative stability was assessed in TAP-deficient T2 cells loaded with the indicated peptides. a Mean fluorescence intensity at flow cytometer indicates binding levels of peptides to HLA molecules. b Decay of mean fluorescence intensity over time indicates stability of the peptide binding to the HLA molecule. MFI₅₀ measures the stability of the binding expressed in hours. c Overlay of the mean fluorescence intensity observed with different concentration of peptides. d PBMCs isolated from HLA-A*02:01 healthy donor were stimulated ex vivo with the indicated peptides for 10 days. Interferon-γ (IFN-γ) secreting T cells were evaluated after in vitro O/N restimulation with individual peptides