Fig. 4

BFE was able to suppress the pro-angiogenic effect of HGF on human endothelial cells: a images taken demonstrating the degree of tubulogenesis/vessel formation by the HECV human endothelial cell line. Using the matrigel-based in vitro angiogenesis model, we demonstrate that stimulation of these cells with HGF resulted in an increased level of vessel formation. Importantly, the addition of BFE in combination with HGF was able to suppress the degree of tubulogenesis. b Images were analysed and the level of vessel formation was quantified. Results confirm that HGF statistically enhanced the level of angiogenesis of these HECV cells and that treatment of these cells with BFE reduced the degree of vessel formation significantly