Fig. 3

BFE and HGF had no effect on breast cancer cell proliferation and the formation of spheroids: a images taken of the degree of spheroid formation within the hanging droplet following 4 days incubation. The BT549 breast cancer cells successfully formed spheroid structures, however there was no obvious differences between the different treatment groups. b The spheroid size was calculated for each group and the results plotted. These results report that HGF and BFE have no impact on the ability of these breast cancer cells to form spheroid structures. c The results from a separate BT549 and MDA-MB-231 proliferation studies also confirm that neither HGF nor BFE have any bearing on the proliferation rate of these breast cancer cell lines